TaqMan RT-PCR Primer/Probe Sets Used for Study of Sphingosine Kinase
Youngleem Kim, Zesheng Wan, C. Patrick Reynolds
Developmental Therapeutics Program
USC-CHLA Institute for Pediatric Clinical Research
The primers were designed using Primer Express V 1.5 software from Applied Biosystems, based on sequences from GenBank/EMBL/DDBJ. Use of thsese primer probe sets will be described in a manuscript in preparation.
human sphingosine kinase 1 (SPHK1: GenBank AF200328)
-forward primer: 5’-GTCACGTGCAGCCCCTTT-3’;
-reverse primer: 5’-CGCGCGTGGTTCCG-3’
-probe: 5’-CTTCACGCTGATGCTCACTGAGCG-3’
human sphingosine kinase 2 gene (SPHK2 GenBank AF245447)
-forward primer: 5’-TGCTCCATGAGGTGCTGAAC-3’
-reverse primer: 5’-GCCCACAGGCATCTTCACA-3’
-probe: 5’-TTCCTCCCAGTCAGGGCGATCTAGGA-3’
human bcl-2 gene (GenBank M14745)
-forward primer: 5’-CATGTGTGTGAGAGCGTCAA-3’
-reverse primer: 5’-AACAGAGGCCGCATGCTG-3’
-probe: 5’-CCTGGTGGACAACATCGCCCTGT-3’
Glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH GeneBank M33197)
-forward primer: 5’-CAACTACATGGTTTACATGTTCCAATATG-3’
-reverse primer: 5’-GGGATCTCGCTCCTGGAAG -3’
-probe: 5’-CGTTCTCAGCCTTGACGGTGC CA -3’