Requesting Cell-lines : List of Available NCI PPTP Lines

The cell lines used for the NCI PPTP in vitro testing panel that are not available from commercial repositories are available via this repository. As for other cell lines in this repository, up to 3 cell lines can be obtained by completing the MTA, between 3 and 10 lines with a short but compelling justification sent with the MTA, and obtaining greater than 10 cell lines requires a brief application reviewed by the COG Translational Research Committee.

Of the 23 PPTP cell lines used for in vitro testing, 8 are available from commercial repositories. Those 8 lines are listed in table B below.

Select the cell lines by clicking on the checkbox for the cell lines you want.

To deselect, just click on the checkbox a second time.

Please visit the COGcell.org website frequently, as cell line data is continually being updated and new cell lines are added.

All cell lines were established in 20% O2 unless otherwise noted. Cell lines ending in "h" designates cell line was established in hypoxic conditions (5% O2 tension).

Table A:  NCI PPTP Cell Lines Available From www.COGcell.org

Rhabdomyosarcoma

Brain Tumor

Ewings Family of Tumors (EFT)

Neuroblastoma

Leukemia

¹ - Phase of therapy for the patient at the time the specimen used to derive the cell line was obtained
Dx (Diagnosis): cell lines derived from samples of patients prior to treatment
PD (Progressive Disease): cell lines derived from samples of patients who relapsed after chemotherapy
PD-BMT: cell lines derived from samples of patients who relapsed after chemotherapy and bone marrow transplantation
PD-PM: cell lines derived from samples of post-mortem patients who relapsed after chemotherapy

² - N-Myc oncogene amplification
N: MYCN Non-Amplified
A: MYCN Amplified

³ - p53 gene function
F: Functional
N: Non-Functional

⁴ - EWS/FLI1 Status
FLI1: friend leukemia insertion - chromosome 11, t(11;22)
ERG: ets-related gene - chromosome 21, t(21;22)

⁵ - Doubling Time

For details on all the cell lines see:

Kang MH, Smith MA, Morton CL, Nino Keshelava N, Houghton PJ, Reynolds CP: National Cancer Institute Pediatric Preclinical Testing Program: Model description for in vitro cytotoxicity testing. Pediatr Blood Cancer 56:239-49, 2011.

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If you are requesting more than 3 cell lines,
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The Disease Committee (for requests of material of a single disease)

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If you are requesting 3-10 cell lines, upload a < 1 page description of research and justification for the lines.

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If you are requesting more than 10 lines, upload a full COG biology specimen application form.

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