Children's Oncology Group (COG) Cell Culture and Xenograft Repository

Requesting Cell-lines

List of Available EFT Lines

How to Request a Cell Line:

(1) Select the cell line(s) by clicking each check box next to the cell line name.
(2) Fill out the institutional or commercial/industry information at the bottom of the page.
(3) Attach a fully signed MTA (including Attachment A)
(4) Attach any additional documents required for your request (i.e. a justification for any request over 5 cell lines total)
(5) Click Submit.

For all other questions regarding your request and/or MTA, please visit our FAQ page.


To view a complete cell line description (origin, growth conditions, pictures, etc.), click on the cell line name to open a pdf data sheet to view and/or download.

Please visit the COGcell.org website frequently, as cell line data is continually being updated and new cell lines are added.


EFT Cell Lines Request Form

Request Cell Line Disease Diagnosis Age (Years) Phase of Therapy1 EWS/FLI1 Status4 p53 Status3 DT5 (hours) Data Sheet
CB-AGPN Ewing's Family of Tumors PNET            
CHLA-9* Ewing's Family of Tumors PNET 14 Dx FLI1 F 51 PDF icon
CHLA-10* Ewing's Family of Tumors PNET 14 PD FLI1 N 32 PDF icon
CHLA-25** Ewing's Family of Tumors PNET 2.6 PD ERG N 99 PDF icon
CHLA-32 Ewing's Family of Tumors PNET 8.5 Dx FLI1 F 26 PDF icon
CHLA-57 Ewing's Family of Tumors PNET            
CHLA-99 Ewing's Family of Tumors Ewing's Sarcoma            
CHLA-218 Ewing's Family of Tumors PNET   PD        
CHLA-258* Ewing's Family of Tumors PNET 14 PD FLI1 N 89 PDF icon
COG-E-352 Ewing's Family of Tumors PNET 17 PD-PM ERG N 28 PDF icon
TC-32 Ewing's Family of Tumors PNET 17 Dx FLI1 N 24 PDF icon
TC-71* Ewing's Family of Tumors Ewing's Sarcoma 22 PD FLI1 N 21 PDF icon
TC-106 Ewing's Family of Tumors Ewing's Sarcoma 19 Dx   F    
TC-138 Ewing's Family of Tumors       FLI1      
TC-205 Ewing's Family of Tumors       FEV      

1 - Phase of therapy for the patient at the time the specimen used to derive the cell line was obtained
Dx (Diagnosis): cell lines derived from samples of patients prior to treatment
PD (Progressive Disease): cell lines derived from samples of patients who relapsed after chemotherapy
PD-BMT: cell lines derived from samples of patients who relapsed after chemotherapy and bone marrow transplantation
PD-PM: cell lines derived from samples of post-mortem patients who relapsed after chemotherapy

3 - p53 gene function
F: Functional
N: Non-Functional

4 - EWS/FLI1 Status
FLI1: friend leukemia insertion - chromosome 11, t(11;22)
ERG: ets-related gene - chromosome 21, t(21;22)

5 - Doubling Time

* - NCI PPTP Cell Lines

** - CHLA-25 (May et al) and TTC-466 (Sorensen et al) were established in two separate laboratories, but from the same specimen obtained from a single biopsy from one patient.

For details on all the NCI PPTP cell lines see:

Kang MH, Smith MA, Morton CL, Nino Keshelava N, Houghton PJ, Reynolds CP: National Cancer Institute Pediatric Preclinical Testing Program: Model description for in vitro cytotoxicity testing. Pediatr Blood Cancer 56:239-49, 2011.

Shipping and Contact Information

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Please provide the name of your courier (eg. FedEx, Airborne, World Courier) if available:
(We cannot ship via UPS or DHL at this time. Please contact us with questions or concerns.)

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If you are requesting more than 5 cell lines,
Who should review the proposal? (Please select one)

The Disease Committee (for requests of material of a single disease)

The Biology and Translational Research Committee (for requests of material from multiple diseases)

If you are requesting 5-15 cell lines, upload a < 1 page description of research and justification for the lines.

(PDF format only; maximum allowed file size is 2 megabytes)

If you are requesting more than 15 lines, upload a full COG biology specimen application form.

(PDF format only; maximum allowed file size is 2 megabytes)

Upload your COG Specimen Transfer Agreement:

(PDF format only; maximum allowed file size is 2 megabytes)

You can also FAX this form to 806-743-2691 if you have difficulty uploading it.

Do you have any special requests or comments? If so, please enter them here.