Children's Oncology Group (COG) Cell Culture and Xenograft Repository

Requesting Cell-lines

List of All Available Lines


How to Request a Cell Line:

(1) Select the cell line(s) by clicking each check box next to the cell line name.
(2) Fill out the institutional or commercial/industry information at the bottom of the page.
(3) Attach a fully signed MTA (including Attachment A)
(4) Attach any additional documents required for your request (i.e. a justification for any request over 5 cell lines total)
(5) Click Submit.

For all other questions regarding your request and/or MTA, please visit our FAQ page.


To view a complete cell line description (origin, growth conditions, pictures, etc.), click on the cell line name to open a pdf data sheet to view and/or download.

All cell lines were established in 20% O2 unless otherwise noted. Cell lines ending in "h" designates cell line was established in hypoxic conditions (5% O2 tension).

Please visit the COGcell.org website frequently, as cell line data is continually being updated and new cell lines are added.


Brain Tumor Lines

Request Cell Line Disease Diagnosis Gender Age (Years) Phase of Therapy1 Site of specimen DT5 (hours) Data Sheet
BT-12* Brain Tumor Atypical teratoid/rhabdoid F 0.2 Dx     PDF icon
CHLA-200 Brain Tumor Glioblastoma multiforme M 12 PD-PM Parietal lobe 54 PDF icon
CHLA-259 Brain Tumor Anaplastic medullablastoma M 14 Dx Posterior fossa 76 PDF icon
CHLA-266* Brain Tumor Atypical teratoid/rhabdoid F 1.5 DX posterior fossa 45 PDF icon
SJ-GBM2* Brain Tumor Glioblastoma multiforme F 5 PD brain   PDF icon

Ewing's Family of Tumors Lines

Request Cell Line Disease Diagnosis Age (Years) Phase of Therapy1 EWS/FLI1 Status4 p53 Status3 DT5 (hours) Data Sheet
CB-AGPN Ewing's Family of Tumors PNET            
CHLA-9* Ewing's Family of Tumors PNET 14 Dx FLI1 F 51 PDF icon
CHLA-10* Ewing's Family of Tumors PNET 14 PD FLI1 N 32 PDF icon
CHLA-25** Ewing's Family of Tumors PNET 2.6 PD ERG N 99 PDF icon
CHLA-32 Ewing's Family of Tumors PNET 8.5 Dx FLI1 F 26 PDF icon
CHLA-57 Ewing's Family of Tumors PNET            
CHLA-99 Ewing's Family of Tumors Ewing's Sarcoma            
CHLA-218 Ewing's Family of Tumors PNET   PD        
CHLA-258* Ewing's Family of Tumors PNET 14 PD FLI1 N 89 PDF icon
COG-E-352 Ewing's Family of Tumors PNET 17 PD-PM ERG N 28 PDF icon
TC-32 Ewing's Family of Tumors PNET 17 Dx FLI1 N 24 PDF icon
TC-71* Ewing's Family of Tumors Ewing's Sarcoma 22 PD FLI1 N 21 PDF icon
TC-106 Ewing's Family of Tumors Ewing's Sarcoma 19 Dx   F    
TC-138 Ewing's Family of Tumors       FLI1      
TC-205 Ewing's Family of Tumors       FEV      

Leukemia Lines

Request Cell Line Disease Diagnosis Gender Age (Years) Phase of Therapy1 Site of Specimen DT5 (hours)
COG-LL-317* Leukemia T-cell ALL M 1.7 PD Bone marrow 30-40
COG-LL-317h* Leukemia T-cell ALL M 1.7 PD Bone marrow 19-20
COG-LL-319h Leukemia Pre-B ALL F 13 Dx Bone marrow 50-62
COG-LL-329h Leukemia T-cell ALL     Dx Bone marrow  
COG-LL-332h Leukemia T-cell ALL M 10 PD Bone marrow 30-34
COG-LL-355h Leukemia Pre-B ALL     Dx Bone marrow  
COG-LL-356h Leukemia Pre-B ALL     Dx Bone marrow  
COG-LL-384h Leukemia T-cell ALL     PD Bone marrow  

Neuroblastoma Lines

Request Cell Line Disease Stage Age (Months) Phase of Therapy1 MYCN Status2 p53 Status3 DT5 (hours) Data Sheet
CHLA-15 Neuroblastoma 4 18 DX N F 21 PDF icon
CHLA-20 Neuroblastoma 4 18 PD N F 28 PDF icon
CHLA-42 Neuroblastoma 4 13 Dx N F   PDF icon
CHLA-90 Neuroblastoma 4 102 PD-BMT N N 59 PDF icon
CHLA-95 Neuroblastoma 4   PD A     PDF icon
CHLA-122 Neuroblastoma 4 24 Dx A F 72 PDF icon
CHLA-136 Neuroblastoma 4 36 PD-BMT A F 44 PDF icon
CHLA-140 Neuroblastoma 4   PD N     PDF icon
CHLA-171 Neuroblastoma 4 101 PD-PM N N   PDF icon
LA-N-5 Neuroblastoma   5 Dx A   100 PDF icon
LA-N-6 Neuroblastoma 4 60 PD N F 150 PDF icon
NB-1643* Neuroblastoma 4 < 36 Dx A     PDF icon
NB-EBc1* Neuroblastoma 4 < 36 PD N     PDF icon
SK-N-BE(1) Neuroblastoma 4 24 Dx A F 96 PDF icon
SK-N-BE(2) Neuroblastoma 4 24 PD A N 27 PDF icon
SK-N-FI Neuroblastoma   132 PD N     PDF icon
SMS-KAN Neuroblastoma 4 36 Dx A F 95 PDF icon
SMS-KANR Neuroblastoma 4 36 PD A F 69 PDF icon
SMS-KCN Neuroblastoma 4 11 Dx A F 109 PDF icon
SMS-KCNR Neuroblastoma 4 11 PD A F 72 PDF icon
SMS-LHN Neuroblastoma 4 24 PD N F 216 PDF icon
SMS-SAN Neuroblastoma 4 36 Dx A F 71 PDF icon
COG-N-291 Neuroblastoma     PD N N 224  
CHLA-53 Neuroblastoma 4   PD-PM A   92  
CHLA-61 Neuroblastoma 4 168 PD-PM N F 102  
CHLA-11 Neuroblastoma 4   PD-BMT,PM A F 53  
CHLA-12 Neuroblastoma 4   PD-BMT,PM A   65  
CHLA-52 Neuroblastoma 4   PD-PM A F 88  
CHLA-60 Neuroblastoma 4 168 PD-PM N F 96  
CHLA-79 Neuroblastoma 4 24 PD-BMT N F    
CHLA-144 Neuroblastoma 4   Dx A      
CHLA-145 Neuroblastoma 4   PD A      
CHLA-172 Neuroblastoma 4   PD-BMT N N    
CHLA-225 Neuroblastoma     PD-PM A N 62  
CHLA-247 Neuroblastoma     PD A      
CHLA-253 Neuroblastoma     PD A      
COG-N-316 Neuroblastoma 4 62 Dx A      
COG-N-321 Neuroblastoma 3 18 PD A N    
COG-N-347 Neuroblastoma 4 24 Dx A   126  
COG-N-399 Neuroblastoma 4 9.6 PD-PM A F 40  
COG-N-415 Neuroblastoma 4 24 PD A N 50  
COG-N-421 Neuroblastoma 4 119 PD-PM A   65  
COG-N-440 Neuroblastoma 4 18 PD-PM A   65  
SK-N-RA Neuroblastoma     PD   F    

Rhabdomyosarcoma Lines

Request Cell Line Disease Diagnosis Age (Years) Phase of Therapy1 p53 Status3 Site of specimen DT5 (hours) Data Sheet
Rh18* Rhabdomyosarcoma embryonal 2 Dx N perineum 65 PDF icon
Rh30* Rhabdomyosarcoma alveolar 17 Dx F bone marrow 35 PDF icon
Rh41* Rhabdomyosarcoma alveolar   PD N lung 60 PDF icon


1 - Phase of therapy for the patient at the time the specimen used to derive the cell line was obtained
Dx (Diagnosis): cell lines derived from samples of patients prior to treatment
PD (Progressive Disease): cell lines derived from samples of patients who relapsed after chemotherapy
PD-BMT: cell lines derived from samples of patients who relapsed after chemotherapy and bone marrow transplantation
PD-PM: cell lines derived from samples of post-mortem patients who relapsed after chemotherapy

2 - N-Myc oncogene amplification
N: MYCN Non-Amplified
A: MYCN Amplified

3 - p53 gene function
F: Functional
N: Non-Functional

4 - EWS/FLI1 Status
FLI1: friend leukemia insertion - chromosome 11, t(11;22)
ERG: ets-related gene - chromosome 21, t(21;22)

5 - Doubling Time

* - NCI PPTP Cell Lines

** - CHLA-25 (May et al) and TTC-466 (Sorensen et al) were established in two separate laboratories, but from the same specimen obtained from a single biopsy from one patient.

For details on all the NCI PPTP cell lines see:

Kang MH, Smith MA, Morton CL, Nino Keshelava N, Houghton PJ, Reynolds CP: National Cancer Institute Pediatric Preclinical Testing Program: Model description for in vitro cytotoxicity testing. Pediatr Blood Cancer 56:239-49, 2011.

Shipping and Contact Information

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If you are requesting more than 5 cell lines:
Who should review the proposal? (Please select one)

The Disease Committee (for requests of material of a single disease)

The Biology and Translational Research Committee (for requests of material from multiple diseases)

If you are requesting 5-15 cell lines, upload a < 1 page description of research and justification for the lines.

(PDF format only; maximum allowed file size is 2 megabytes)

If you are requesting more than 15 lines, upload a full COG biology specimen application form.

(PDF format only; maximum allowed file size is 2 megabytes)

Upload your COG Specimen Transfer Agreement:

(PDF format only; maximum allowed file size is 2 megabytes)

You can also FAX this form to 806-743-2691 if you have difficulty uploading it.

Do you have any special requests or comments? If so, please enter them here.